EXPERIMENTAL CANCER CHEMOTHRAPY RESEARCH LAB. Co., Ltd.
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Company Feature

1) The characteristic of human tumor xenografts

  1. The cages in large vinyl-isolator The human tumors had been excised surgically from cancer patients and established as human tumor xenografts (HTXs) under specific pathogen free condition in nude mice.
    Fifty HTXs had been established: gastric HTXS (12), colon (7), breast (6), pancreas (3), esophagus (2), liver (2), renal (3), bile duct (1), urinary duct (1), ovary (2), uterus (2), bladder (2), testis (1), melanoma (2) have been passaged in nude mice of the same sex as the donor patients within vinyl-isolator since 1976.
    link to http://www.eccr-lab.co.jp/gan-list.html
    HTXs have been maintainted in large vinyl-isolator.
  2. The biological characteristics of those 50 HTXs have been identified. As passaged in vivo, original characteristic features have been maintained. As to 40 out of 50 HTXs, their genes also have been analyzed.
  3. Effectiveness of given antitumor drugs (16) to the above-mentioned HTXs has been examined. The HTXs from the same organ shows different sensitivity, their characteristics are maintained if xenografts are passaged in vivo.
  4. Antitumor effectivities between HTX in nude mice and original cancer in patient were found to be excellently correlative.

2) The advantage of our model system

  1. Clean bench and vinyl-isolator method
    Clean bench and vinyl-isolator methodAnimals (including human tumor transplanted animals) are always kept in a vinyl-isolator, except for tumor transplantation and drug administration that were done in clean bench (class 100).
    The temperature and humidity in the isolator are 23±2℃ and 50±10%, respectively so that the long period experiment can be performed without infection. The drug administration and treatment schedule can be adapted to clinical ones. The consecutive oral administration or intermittent intravenous administration is available.
  2. (1) Antitumor effect will be estimated by tumor growth inhibition ratio (IR) calculated by the mean tumor weights of treated animals and untreated control animals. Student’s t test (both sides) will be used as a statistical analysis. (2) In addition to IR, if you want, pathological changes (classificatory criteria by Drs.　Oboshi and Shimosato Lit.1) for tumor in treated animals could be examined. Lit.1: Y. Shimosato, S. Oboshi, K. Baba, Histological evaluation of effects of radiotherapy and chemotherapy for carcinomas, JPN J. Clin. Oncol. 1971;1:19-35.
    (3) In addition to routine observation (body weight change, disease symptoms), blood test will be conducted to estimate side effects (option).

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